Construct: shRNA TRCN0000238429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011878.2-344s21c1
DNA Barcode:
GGATCCAAGAATCATAGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tiam2 (24001)
Gene Description:
T cell lymphoma invasion and metastasis 2
Transcript:
RefSeq NM_011878.2 (CURRENT)
Match location:
Position 344 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATCCAAGAATCATAGTAAT
Hairpin Sequence:
5'-CCGG-GGATCCAAGAATCATAGTAAT-CTCGAG-ATTACTATGATTCTTGGATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATCCAAGAATCATAGTAATCTCGAGATTACTATGATTCTTGGATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATCCAAGAATCATAGTAATCTCGAGATTACTATGATTCTTGGATCC-3'

Other clones with same target sequence:

(none)