Construct: shRNA TRCN0000238493

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201529.2-4477s21c1
DNA Barcode:
GTACCGAAGGCCCATTGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lmo7 (380928)
Gene Description:
LIM domain only 7
Transcript:
RefSeq NM_201529.2 (CURRENT)
Match location:
Position 4477 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238493)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACCGAAGGCCCATTGATTC
Hairpin Sequence:
5'-CCGG-GTACCGAAGGCCCATTGATTC-CTCGAG-GAATCAATGGGCCTTCGGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACCGAAGGCCCATTGATTCCTCGAGGAATCAATGGGCCTTCGGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACCGAAGGCCCATTGATTCCTCGAGGAATCAATGGGCCTTCGGTAC-3'

Other clones with same target sequence:

(none)