Construct: shRNA TRCN0000238593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_137597.7-885s21c1
DNA Barcode:
CAAAGTGTGTGGGAAGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm12258 (237769)
Gene Description:
predicted gene 12258
Transcript:
RefSeq XM_137597.7 (NON-CURRENT)
Match location:
Position 885 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGTGTGTGGGAAGTTATT
Hairpin Sequence:
5'-CCGG-CAAAGTGTGTGGGAAGTTATT-CTCGAG-AATAACTTCCCACACACTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGTGTGTGGGAAGTTATTCTCGAGAATAACTTCCCACACACTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGTGTGTGGGAAGTTATTCTCGAGAATAACTTCCCACACACTTTG-3'

Other clones with same target sequence:

(none)