Construct: shRNA TRCN0000238606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_123229.6-870s21c1
DNA Barcode:
TGAGATTATTTGTGCGAATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm4835 (225228)
Gene Description:
predicted pseudogene 4835; mortality factor 4 like 1 pseudogene
Transcript:
RefSeq XM_123229.6 (NON-CURRENT)
Match location:
Position 870 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGATTATTTGTGCGAATTG
Hairpin Sequence:
5'-CCGG-TGAGATTATTTGTGCGAATTG-CTCGAG-CAATTCGCACAAATAATCTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGATTATTTGTGCGAATTGCTCGAGCAATTCGCACAAATAATCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGATTATTTGTGCGAATTGCTCGAGCAATTCGCACAAATAATCTCA-3'

Other clones with same target sequence:

(none)