Construct: shRNA TRCN0000238646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028536.1-2417s21c1
DNA Barcode:
CTGAGCATTCCCGTGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccsap (73420)
Gene Description:
centriole, cilia and spindle associated protein
Transcript:
RefSeq NM_028536.1 (CURRENT)
Match location:
Position 2417 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGCATTCCCGTGATATTT
Hairpin Sequence:
5'-CCGG-CTGAGCATTCCCGTGATATTT-CTCGAG-AAATATCACGGGAATGCTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGCATTCCCGTGATATTTCTCGAGAAATATCACGGGAATGCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGCATTCCCGTGATATTTCTCGAGAAATATCACGGGAATGCTCAG-3'

Other clones with same target sequence:

(none)