Construct: shRNA TRCN0000238684

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_130416.6-303s21c1
DNA Barcode:
ATCTGATACCAACCGTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gins1 (69270)
Gene Description:
GINS complex subunit 1 (Psf1 homolog)
Transcript:
RefSeq XM_130416.6 (NON-CURRENT)
Match location:
Position 303 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238684)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTGATACCAACCGTCAAAT
Hairpin Sequence:
5'-CCGG-ATCTGATACCAACCGTCAAAT-CTCGAG-ATTTGACGGTTGGTATCAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTGATACCAACCGTCAAATCTCGAGATTTGACGGTTGGTATCAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTGATACCAACCGTCAAATCTCGAGATTTGACGGTTGGTATCAGAT-3'

Other clones with same target sequence:

(none)