Construct: shRNA TRCN0000238699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_981688.1-201s21c1
DNA Barcode:
ACCGATCCTCCGTCAACATCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rundc2a (385605)
Gene Description:
RUN domain containing 2A
Transcript:
RefSeq XM_981688.1 (NON-CURRENT)
Match location:
Position 201 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCGATCCTCCGTCAACATCA
Hairpin Sequence:
5'-CCGG-ACCGATCCTCCGTCAACATCA-CTCGAG-TGATGTTGACGGAGGATCGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCGATCCTCCGTCAACATCACTCGAGTGATGTTGACGGAGGATCGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCGATCCTCCGTCAACATCACTCGAGTGATGTTGACGGAGGATCGGT-3'

Other clones with same target sequence:

(none)