Construct: shRNA TRCN0000238762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_125512.4-939s21c1
DNA Barcode:
GAGCCTCTATGTGGTAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zc2hc1b (75122)
Gene Description:
zinc finger, C2HC-type containing 1B
Transcript:
RefSeq XM_125512.4 (NON-CURRENT)
Match location:
Position 939 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCCTCTATGTGGTAGTTAA
Hairpin Sequence:
5'-CCGG-GAGCCTCTATGTGGTAGTTAA-CTCGAG-TTAACTACCACATAGAGGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCCTCTATGTGGTAGTTAACTCGAGTTAACTACCACATAGAGGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCCTCTATGTGGTAGTTAACTCGAGTTAACTACCACATAGAGGCTC-3'

Other clones with same target sequence:

(none)