Construct: shRNA TRCN0000238767

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010093.3-803s21c1
DNA Barcode:
GTACCTCTCAGATGGTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
E2f3 (13557)
Gene Description:
E2F transcription factor 3
Transcript:
RefSeq NM_010093.3 (CURRENT)
Match location:
Position 803 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238767)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACCTCTCAGATGGTCTAAA
Hairpin Sequence:
5'-CCGG-GTACCTCTCAGATGGTCTAAA-CTCGAG-TTTAGACCATCTGAGAGGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACCTCTCAGATGGTCTAAACTCGAGTTTAGACCATCTGAGAGGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACCTCTCAGATGGTCTAAACTCGAGTTTAGACCATCTGAGAGGTAC-3'

Other clones with same target sequence:

(none)