Construct: shRNA TRCN0000238794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005407.1-1407s21c1
DNA Barcode:
CCGCTTCTGTGCCAAAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SALL2 (6297)
Gene Description:
spalt like transcription factor 2
Transcript:
RefSeq NM_005407.1 (NON-CURRENT)
Match location:
Position 1407 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTTCTGTGCCAAAGTATT
Hairpin Sequence:
5'-CCGG-CCGCTTCTGTGCCAAAGTATT-CTCGAG-AATACTTTGGCACAGAAGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTTCTGTGCCAAAGTATTCTCGAGAATACTTTGGCACAGAAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTTCTGTGCCAAAGTATTCTCGAGAATACTTTGGCACAGAAGCGG-3'

Other clones with same target sequence:

(none)