Construct: shRNA TRCN0000238805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015941.2-864s21c1
DNA Barcode:
CAAGTGATAGTTCGCAGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V1H (51606)
Gene Description:
ATPase H+ transporting V1 subunit H
Transcript:
RefSeq NM_015941.2 (NON-CURRENT)
Match location:
Position 864 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTGATAGTTCGCAGTATG
Hairpin Sequence:
5'-CCGG-CAAGTGATAGTTCGCAGTATG-CTCGAG-CATACTGCGAACTATCACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTGATAGTTCGCAGTATGCTCGAGCATACTGCGAACTATCACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTGATAGTTCGCAGTATGCTCGAGCATACTGCGAACTATCACTTG-3'

Other clones with same target sequence:

(none)