Construct: shRNA TRCN0000238857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001474507.1-657s21c1
DNA Barcode:
TATGGAGAGGAAGATTGAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp746 (69228)
Gene Description:
zinc finger protein 746
Transcript:
RefSeq XM_001474507.1 (NON-CURRENT)
Match location:
Position 657 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGGAGAGGAAGATTGAATC
Hairpin Sequence:
5'-CCGG-TATGGAGAGGAAGATTGAATC-CTCGAG-GATTCAATCTTCCTCTCCATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGGAGAGGAAGATTGAATCCTCGAGGATTCAATCTTCCTCTCCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGGAGAGGAAGATTGAATCCTCGAGGATTCAATCTTCCTCTCCATA-3'

Other clones with same target sequence:

(none)