Construct: shRNA TRCN0000238901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001000101.2-2548s21c1
DNA Barcode:
CATTTGGCAGCTGGTTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnks2 (74493)
Gene Description:
tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
Transcript:
RefSeq XM_001000101.2 (NON-CURRENT)
Match location:
Position 2548 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTTGGCAGCTGGTTATAAT
Hairpin Sequence:
5'-CCGG-CATTTGGCAGCTGGTTATAAT-CTCGAG-ATTATAACCAGCTGCCAAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTTGGCAGCTGGTTATAATCTCGAGATTATAACCAGCTGCCAAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTTGGCAGCTGGTTATAATCTCGAGATTATAACCAGCTGCCAAATG-3'

Other clones with same target sequence:

(none)