Construct: shRNA TRCN0000238937

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081383.1-15461s21c1
DNA Barcode:
GATAGAGCTAAGGGATAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kmt2c (231051)
Gene Description:
lysine (K)-specific methyltransferase 2C
Transcript:
RefSeq NM_001081383.1 (CURRENT)
Match location:
Position 15461 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238937)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAGAGCTAAGGGATAATAA
Hairpin Sequence:
5'-CCGG-GATAGAGCTAAGGGATAATAA-CTCGAG-TTATTATCCCTTAGCTCTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAGAGCTAAGGGATAATAACTCGAGTTATTATCCCTTAGCTCTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAGAGCTAAGGGATAATAACTCGAGTTATTATCCCTTAGCTCTATC-3'

Other clones with same target sequence:

(none)