Construct: shRNA TRCN0000238943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027742.2-2336s21c1
DNA Barcode:
CGCCTGAATCTACCTACTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrfip2 (71268)
Gene Description:
leucine rich repeat (in FLII) interacting protein 2
Transcript:
RefSeq NM_027742.2 (NON-CURRENT)
Match location:
Position 2336 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTGAATCTACCTACTAAA
Hairpin Sequence:
5'-CCGG-CGCCTGAATCTACCTACTAAA-CTCGAG-TTTAGTAGGTAGATTCAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTGAATCTACCTACTAAACTCGAGTTTAGTAGGTAGATTCAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTGAATCTACCTACTAAACTCGAGTTTAGTAGGTAGATTCAGGCG-3'

Other clones with same target sequence:

(none)