Construct: shRNA TRCN0000238949

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001472312.1-2078s21c1
DNA Barcode:
GCATGTACTGGATCGGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
6720489N17Rik (211378)
Gene Description:
RIKEN cDNA 6720489N17 gene
Transcript:
RefSeq XM_001472312.1 (NON-CURRENT)
Match location:
Position 2078 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238949)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTACTGGATCGGTTAAA
Hairpin Sequence:
5'-CCGG-GCATGTACTGGATCGGTTAAA-CTCGAG-TTTAACCGATCCAGTACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTACTGGATCGGTTAAACTCGAGTTTAACCGATCCAGTACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTACTGGATCGGTTAAACTCGAGTTTAACCGATCCAGTACATGC-3'

Other clones with same target sequence:

(none)