Construct: shRNA TRCN0000238953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_976217.1-1359s21c1
DNA Barcode:
ACCTCCTAGTGTTGGGTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp407 (240476)
Gene Description:
zinc finger protein 407
Transcript:
RefSeq XM_976217.1 (NON-CURRENT)
Match location:
Position 1359 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTCCTAGTGTTGGGTAATA
Hairpin Sequence:
5'-CCGG-ACCTCCTAGTGTTGGGTAATA-CTCGAG-TATTACCCAACACTAGGAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTCCTAGTGTTGGGTAATACTCGAGTATTACCCAACACTAGGAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTCCTAGTGTTGGGTAATACTCGAGTATTACCCAACACTAGGAGGT-3'

Other clones with same target sequence:

(none)