Construct: shRNA TRCN0000238970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_132089.7-3818s21c1
DNA Barcode:
ACTCGATGGACAACGATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgra3 (70693)
Gene Description:
adhesion G protein-coupled receptor A3
Transcript:
RefSeq XM_132089.7 (NON-CURRENT)
Match location:
Position 3818 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCGATGGACAACGATATTA
Hairpin Sequence:
5'-CCGG-ACTCGATGGACAACGATATTA-CTCGAG-TAATATCGTTGTCCATCGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCGATGGACAACGATATTACTCGAGTAATATCGTTGTCCATCGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCGATGGACAACGATATTACTCGAGTAATATCGTTGTCCATCGAGT-3'

Other clones with same target sequence:

(none)