Construct: shRNA TRCN0000239022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081141.1-3348s21c1
DNA Barcode:
AGGAGTTGGGATGTCAGAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabbr2 (242425)
Gene Description:
gamma-aminobutyric acid (GABA) B receptor, 2
Transcript:
RefSeq NM_001081141.1 (CURRENT)
Match location:
Position 3348 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGTTGGGATGTCAGAAAC
Hairpin Sequence:
5'-CCGG-AGGAGTTGGGATGTCAGAAAC-CTCGAG-GTTTCTGACATCCCAACTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGTTGGGATGTCAGAAACCTCGAGGTTTCTGACATCCCAACTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGTTGGGATGTCAGAAACCTCGAGGTTTCTGACATCCCAACTCCT-3'

Other clones with same target sequence:

(none)