Construct: shRNA TRCN0000239063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001123362.1-480s21c1
DNA Barcode:
TTCAACGAGGATGGTACAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prdm12 (381359)
Gene Description:
PR domain containing 12
Transcript:
RefSeq NM_001123362.1 (CURRENT)
Match location:
Position 480 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCAACGAGGATGGTACAGTA
Hairpin Sequence:
5'-CCGG-TTCAACGAGGATGGTACAGTA-CTCGAG-TACTGTACCATCCTCGTTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCAACGAGGATGGTACAGTACTCGAGTACTGTACCATCCTCGTTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCAACGAGGATGGTACAGTACTCGAGTACTGTACCATCCTCGTTGAA-3'

Other clones with same target sequence:

(none)