Construct: shRNA TRCN0000239102

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099624.2-5997s21c1
DNA Barcode:
GATCAGTAGATAAACGTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rapgef2 (76089)
Gene Description:
Rap guanine nucleotide exchange factor (GEF) 2
Transcript:
RefSeq NM_001099624.2 (NON-CURRENT)
Match location:
Position 5997 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239102)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAGTAGATAAACGTAAAT
Hairpin Sequence:
5'-CCGG-GATCAGTAGATAAACGTAAAT-CTCGAG-ATTTACGTTTATCTACTGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAGTAGATAAACGTAAATCTCGAGATTTACGTTTATCTACTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAGTAGATAAACGTAAATCTCGAGATTTACGTTTATCTACTGATC-3'

Other clones with same target sequence:

(none)