Construct: shRNA TRCN0000239116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001077354.2-2583s21c1
DNA Barcode:
TCGCAAAGAAGACGGTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C77370 (245555)
Gene Description:
expressed sequence C77370
Transcript:
RefSeq NM_001077354.2 (CURRENT)
Match location:
Position 2583 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCAAAGAAGACGGTGTAAA
Hairpin Sequence:
5'-CCGG-TCGCAAAGAAGACGGTGTAAA-CTCGAG-TTTACACCGTCTTCTTTGCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCAAAGAAGACGGTGTAAACTCGAGTTTACACCGTCTTCTTTGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCAAAGAAGACGGTGTAAACTCGAGTTTACACCGTCTTCTTTGCGA-3'

Other clones with same target sequence:

(none)