Construct: shRNA TRCN0000239121

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033425.3-2528s21c1
DNA Barcode:
ACCCATGTTCCAGCAACCTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zscan10 (332221)
Gene Description:
zinc finger and SCAN domain containing 10
Transcript:
RefSeq NM_001033425.3 (NON-CURRENT)
Match location:
Position 2528 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239121)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCATGTTCCAGCAACCTTG
Hairpin Sequence:
5'-CCGG-ACCCATGTTCCAGCAACCTTG-CTCGAG-CAAGGTTGCTGGAACATGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCATGTTCCAGCAACCTTGCTCGAGCAAGGTTGCTGGAACATGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCATGTTCCAGCAACCTTGCTCGAGCAAGGTTGCTGGAACATGGGT-3'

Other clones with same target sequence:

(none)