Construct: shRNA TRCN0000239230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029922.2-2426s21c1
DNA Barcode:
GGATCCATCTGCCCTTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Parp6 (67287)
Gene Description:
poly (ADP-ribose) polymerase family, member 6
Transcript:
RefSeq NM_029922.2 (NON-CURRENT)
Match location:
Position 2426 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATCCATCTGCCCTTGTAAA
Hairpin Sequence:
5'-CCGG-GGATCCATCTGCCCTTGTAAA-CTCGAG-TTTACAAGGGCAGATGGATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATCCATCTGCCCTTGTAAACTCGAGTTTACAAGGGCAGATGGATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATCCATCTGCCCTTGTAAACTCGAGTTTACAAGGGCAGATGGATCC-3'

Other clones with same target sequence:

(none)