Construct: shRNA TRCN0000239248

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153399.2-1487s21c1
DNA Barcode:
GGAAACAAGTGGAGCATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syne1 (64009)
Gene Description:
spectrin repeat containing, nuclear envelope 1
Transcript:
RefSeq NM_153399.2 (CURRENT)
Match location:
Position 1487 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239248)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAAACAAGTGGAGCATATAA
Hairpin Sequence:
5'-CCGG-GGAAACAAGTGGAGCATATAA-CTCGAG-TTATATGCTCCACTTGTTTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAAACAAGTGGAGCATATAACTCGAGTTATATGCTCCACTTGTTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAAACAAGTGGAGCATATAACTCGAGTTATATGCTCCACTTGTTTCC-3'

Other clones with same target sequence:

(none)