Construct: shRNA TRCN0000239268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_140451.7-10164s21c1
DNA Barcode:
CACACAACACTTTAGTTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lama3 (16774)
Gene Description:
laminin, alpha 3
Transcript:
RefSeq XM_140451.7 (NON-CURRENT)
Match location:
Position 10164 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACACAACACTTTAGTTAATA
Hairpin Sequence:
5'-CCGG-CACACAACACTTTAGTTAATA-CTCGAG-TATTAACTAAAGTGTTGTGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACACAACACTTTAGTTAATACTCGAGTATTAACTAAAGTGTTGTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACACAACACTTTAGTTAATACTCGAGTATTAACTAAAGTGTTGTGTG-3'

Other clones with same target sequence:

(none)