Construct: shRNA TRCN0000239270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_140451.7-8642s21c1
DNA Barcode:
TCAGCATGGGAGGCGGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lama3 (16774)
Gene Description:
laminin, alpha 3
Transcript:
RefSeq XM_140451.7 (NON-CURRENT)
Match location:
Position 8642 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGCATGGGAGGCGGTTATT
Hairpin Sequence:
5'-CCGG-TCAGCATGGGAGGCGGTTATT-CTCGAG-AATAACCGCCTCCCATGCTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGCATGGGAGGCGGTTATTCTCGAGAATAACCGCCTCCCATGCTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGCATGGGAGGCGGTTATTCTCGAGAATAACCGCCTCCCATGCTGA-3'

Other clones with same target sequence:

(none)