Construct: shRNA TRCN0000239297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015168.1-928s21c1
DNA Barcode:
CAATGGGAGACGACGACTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZC3H4 (23211)
Gene Description:
zinc finger CCCH-type containing 4
Transcript:
RefSeq NM_015168.1 (NON-CURRENT)
Match location:
Position 928 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGGGAGACGACGACTATG
Hairpin Sequence:
5'-CCGG-CAATGGGAGACGACGACTATG-CTCGAG-CATAGTCGTCGTCTCCCATTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGGGAGACGACGACTATGCTCGAGCATAGTCGTCGTCTCCCATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGGGAGACGACGACTATGCTCGAGCATAGTCGTCGTCTCCCATTG-3'

Other clones with same target sequence:

(none)