Construct: shRNA TRCN0000239316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015164.1-2987s21c1
DNA Barcode:
TCACGGATGACCGCCTCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHM2 (23207)
Gene Description:
pleckstrin homology and RUN domain containing M2
Transcript:
RefSeq NM_015164.1 (NON-CURRENT)
Match location:
Position 2987 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACGGATGACCGCCTCTTTA
Hairpin Sequence:
5'-CCGG-TCACGGATGACCGCCTCTTTA-CTCGAG-TAAAGAGGCGGTCATCCGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACGGATGACCGCCTCTTTACTCGAGTAAAGAGGCGGTCATCCGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACGGATGACCGCCTCTTTACTCGAGTAAAGAGGCGGTCATCCGTGA-3'

Other clones with same target sequence:

(none)