Construct: shRNA TRCN0000239358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080410.1-1952s21c1
DNA Barcode:
CAAGGGTGCTGCTTGAGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO41 (150726)
Gene Description:
F-box protein 41
Transcript:
RefSeq NM_001080410.1 (NON-CURRENT)
Match location:
Position 1952 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGGTGCTGCTTGAGAATG
Hairpin Sequence:
5'-CCGG-CAAGGGTGCTGCTTGAGAATG-CTCGAG-CATTCTCAAGCAGCACCCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGGTGCTGCTTGAGAATGCTCGAGCATTCTCAAGCAGCACCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGGTGCTGCTTGAGAATGCTCGAGCATTCTCAAGCAGCACCCTTG-3'

Other clones with same target sequence:

(none)