Construct: shRNA TRCN0000239366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025185.3-2959s21c1
DNA Barcode:
GCCAGCAGCAAGGAGTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TANC2 (26115)
Gene Description:
tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
Transcript:
RefSeq NM_025185.3 (NON-CURRENT)
Match location:
Position 2959 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCAGCAAGGAGTATTTA
Hairpin Sequence:
5'-CCGG-GCCAGCAGCAAGGAGTATTTA-CTCGAG-TAAATACTCCTTGCTGCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCAGCAAGGAGTATTTACTCGAGTAAATACTCCTTGCTGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCAGCAAGGAGTATTTACTCGAGTAAATACTCCTTGCTGCTGGC-3'

Other clones with same target sequence:

(none)