Construct: shRNA TRCN0000239386

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001716253.1-2879s21c1
DNA Barcode:
TCAATAGTGAGCATAAGATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM66 (9866)
Gene Description:
tripartite motif containing 66
Transcript:
RefSeq XM_001716253.1 (NON-CURRENT)
Match location:
Position 2879 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239386)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAATAGTGAGCATAAGATTC
Hairpin Sequence:
5'-CCGG-TCAATAGTGAGCATAAGATTC-CTCGAG-GAATCTTATGCTCACTATTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAATAGTGAGCATAAGATTCCTCGAGGAATCTTATGCTCACTATTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAATAGTGAGCATAAGATTCCTCGAGGAATCTTATGCTCACTATTGA-3'

Other clones with same target sequence:

(none)