Construct: shRNA TRCN0000239397
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_899317.3-442s21c1
- DNA Barcode:
- ACCTTCTGGGATCCGTGACAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- 2810047C21Rik1 (72716)
- Gene Description:
- RIKEN cDNA 2810047C21 gene 1
- Transcript:
- RefSeq XM_899317.3 (NON-CURRENT)
- Match location:
- Position 442 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000239397)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACCTTCTGGGATCCGTGACAT
- Hairpin Sequence:
-
5'-CCGG-ACCTTCTGGGATCCGTGACAT-CTCGAG-ATGTCACGGATCCCAGAAGGT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACCTTCTGGGATCCGTGACATCTCGAGATGTCACGGATCCCAGAAGGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACCTTCTGGGATCCGTGACATCTCGAGATGTCACGGATCCCAGAAGGT-3'
Other clones with same target sequence:
(none)
