Construct: shRNA TRCN0000239401

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_899317.3-271s21c1
DNA Barcode:
TTTCCCAGTATTCAGTGAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2810047C21Rik1 (72716)
Gene Description:
RIKEN cDNA 2810047C21 gene 1
Transcript:
RefSeq XM_899317.3 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239401)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTCCCAGTATTCAGTGAAAC
Hairpin Sequence:
5'-CCGG-TTTCCCAGTATTCAGTGAAAC-CTCGAG-GTTTCACTGAATACTGGGAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTCCCAGTATTCAGTGAAACCTCGAGGTTTCACTGAATACTGGGAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTCCCAGTATTCAGTGAAACCTCGAGGTTTCACTGAATACTGGGAAA-3'

Other clones with same target sequence:

(none)