Construct: shRNA TRCN0000239437

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001045514.2-2580s21c1
DNA Barcode:
GAGATACCTCAGCGTTAAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akna (100182)
Gene Description:
AT-hook transcription factor
Transcript:
RefSeq NM_001045514.2 (NON-CURRENT)
Match location:
Position 2580 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239437)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATACCTCAGCGTTAAATC
Hairpin Sequence:
5'-CCGG-GAGATACCTCAGCGTTAAATC-CTCGAG-GATTTAACGCTGAGGTATCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATACCTCAGCGTTAAATCCTCGAGGATTTAACGCTGAGGTATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATACCTCAGCGTTAAATCCTCGAGGATTTAACGCTGAGGTATCTC-3'

Other clones with same target sequence:

(none)