Construct: shRNA TRCN0000239610

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001723798.1-2266s21c1
DNA Barcode:
TATGGGTCATATCTCTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF860 (344787)
Gene Description:
zinc finger protein 860
Transcript:
RefSeq XM_001723798.1 (NON-CURRENT)
Match location:
Position 2266 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239610)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGGGTCATATCTCTATTTA
Hairpin Sequence:
5'-CCGG-TATGGGTCATATCTCTATTTA-CTCGAG-TAAATAGAGATATGACCCATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGGGTCATATCTCTATTTACTCGAGTAAATAGAGATATGACCCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGGGTCATATCTCTATTTACTCGAGTAAATAGAGATATGACCCATA-3'

Other clones with same target sequence:

(none)