Construct: shRNA TRCN0000239657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021269.2-517s21c1
DNA Barcode:
GGATATCAGAAAGGCTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF708 (7562)
Gene Description:
zinc finger protein 708
Transcript:
RefSeq NM_021269.2 (NON-CURRENT)
Match location:
Position 517 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATATCAGAAAGGCTGTAAA
Hairpin Sequence:
5'-CCGG-GGATATCAGAAAGGCTGTAAA-CTCGAG-TTTACAGCCTTTCTGATATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATATCAGAAAGGCTGTAAACTCGAGTTTACAGCCTTTCTGATATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATATCAGAAAGGCTGTAAACTCGAGTTTACAGCCTTTCTGATATCC-3'

Other clones with same target sequence:

(none)