Construct: shRNA TRCN0000239670

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_488281.4-1641s21c1
DNA Barcode:
TCCTCATCTCTTCAAGTTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B020011L13Rik (547097)
Gene Description:
RIKEN cDNA B020011L13 gene
Transcript:
RefSeq XM_488281.4 (NON-CURRENT)
Match location:
Position 1641 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239670)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTCATCTCTTCAAGTTTAC
Hairpin Sequence:
5'-CCGG-TCCTCATCTCTTCAAGTTTAC-CTCGAG-GTAAACTTGAAGAGATGAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTCATCTCTTCAAGTTTACCTCGAGGTAAACTTGAAGAGATGAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTCATCTCTTCAAGTTTACCTCGAGGTAAACTTGAAGAGATGAGGA-3'

Other clones with same target sequence:

(none)