Construct: shRNA TRCN0000239756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_894989.3-40s21c1
DNA Barcode:
ACAGGAGAGAAGCCATATAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm11677 (629967)
Gene Description:
predicted gene 11677
Transcript:
RefSeq XM_894989.3 (NON-CURRENT)
Match location:
Position 40 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGGAGAGAAGCCATATAAG
Hairpin Sequence:
5'-CCGG-ACAGGAGAGAAGCCATATAAG-CTCGAG-CTTATATGGCTTCTCTCCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGGAGAGAAGCCATATAAGCTCGAGCTTATATGGCTTCTCTCCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGGAGAGAAGCCATATAAGCTCGAGCTTATATGGCTTCTCTCCTGT-3'

Other clones with same target sequence:

(none)