Construct: shRNA TRCN0000239774

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_914561.3-51s21c1
DNA Barcode:
TCCCAACTTGGGTCCTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC638517 (638517)
Gene Description:
cyclic AMP-responsive element-binding protein 3-like protein 2-like
Transcript:
RefSeq XM_914561.3 (NON-CURRENT)
Match location:
Position 51 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239774)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCAACTTGGGTCCTGTAAA
Hairpin Sequence:
5'-CCGG-TCCCAACTTGGGTCCTGTAAA-CTCGAG-TTTACAGGACCCAAGTTGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCAACTTGGGTCCTGTAAACTCGAGTTTACAGGACCCAAGTTGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCAACTTGGGTCCTGTAAACTCGAGTTTACAGGACCCAAGTTGGGA-3'

Other clones with same target sequence:

(none)