Construct: shRNA TRCN0000239788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_908118.3-809s21c1
DNA Barcode:
CAGGAGAGACACCTTACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC633654 (633654)
Gene Description:
zinc finger protein 41-like
Transcript:
RefSeq XM_908118.3 (NON-CURRENT)
Match location:
Position 809 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAGAGACACCTTACAAAT
Hairpin Sequence:
5'-CCGG-CAGGAGAGACACCTTACAAAT-CTCGAG-ATTTGTAAGGTGTCTCTCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAGAGACACCTTACAAATCTCGAGATTTGTAAGGTGTCTCTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAGAGACACCTTACAAATCTCGAGATTTGTAAGGTGTCTCTCCTG-3'

Other clones with same target sequence:

(none)