Construct: shRNA TRCN0000239830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_915944.3-229s21c1
DNA Barcode:
GCAGGTTGGCTGCAATCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC639496 (639496)
Gene Description:
similar to synovial sarcoma, X member B, breakpoint 10
Transcript:
RefSeq XM_915944.3 (NON-CURRENT)
Match location:
Position 229 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTTGGCTGCAATCTAAT
Hairpin Sequence:
5'-CCGG-GCAGGTTGGCTGCAATCTAAT-CTCGAG-ATTAGATTGCAGCCAACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTTGGCTGCAATCTAATCTCGAGATTAGATTGCAGCCAACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTTGGCTGCAATCTAATCTCGAGATTAGATTGCAGCCAACCTGC-3'

Other clones with same target sequence:

(none)