Construct: shRNA TRCN0000239891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_894313.3-505s21c1
DNA Barcode:
CATGAACAGAGTTGAAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Morf4l1b (627352)
Gene Description:
mortality factor 4 like 1B
Transcript:
RefSeq XM_894313.3 (NON-CURRENT)
Match location:
Position 505 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAACAGAGTTGAAGTTAA
Hairpin Sequence:
5'-CCGG-CATGAACAGAGTTGAAGTTAA-CTCGAG-TTAACTTCAACTCTGTTCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAACAGAGTTGAAGTTAACTCGAGTTAACTTCAACTCTGTTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAACAGAGTTGAAGTTAACTCGAGTTAACTTCAACTCTGTTCATG-3'

Other clones with same target sequence:

(none)