Construct: shRNA TRCN0000239946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_905848.2-545s21c1
DNA Barcode:
TGGGAAATTCTCTACAATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm7075 (631906)
Gene Description:
predicted gene 7075; ring finger protein 7 pseudogene
Transcript:
RefSeq XM_905848.2 (NON-CURRENT)
Match location:
Position 545 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGAAATTCTCTACAATTAA
Hairpin Sequence:
5'-CCGG-TGGGAAATTCTCTACAATTAA-CTCGAG-TTAATTGTAGAGAATTTCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGAAATTCTCTACAATTAACTCGAGTTAATTGTAGAGAATTTCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGAAATTCTCTACAATTAACTCGAGTTAATTGTAGAGAATTTCCCA-3'

Other clones with same target sequence:

(none)