Construct: shRNA TRCN0000239982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081565.2-316s21c1
DNA Barcode:
AGCTTGAAGAAGACAAGATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ssxb8 (631002)
Gene Description:
synovial sarcoma, X member B8
Transcript:
RefSeq NM_001081565.2 (NON-CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000239982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTTGAAGAAGACAAGATTG
Hairpin Sequence:
5'-CCGG-AGCTTGAAGAAGACAAGATTG-CTCGAG-CAATCTTGTCTTCTTCAAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTTGAAGAAGACAAGATTGCTCGAGCAATCTTGTCTTCTTCAAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTTGAAGAAGACAAGATTGCTCGAGCAATCTTGTCTTCTTCAAGCT-3'

Other clones with same target sequence:

(none)