Construct: shRNA TRCN0000240022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_908293.2-782s21c1
DNA Barcode:
ACTGCACTGCACACGCAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC633778 (633778)
Gene Description:
similar to glucagon-like peptide 2 receptor
Transcript:
RefSeq XM_908293.2 (NON-CURRENT)
Match location:
Position 782 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGCACTGCACACGCAATTA
Hairpin Sequence:
5'-CCGG-ACTGCACTGCACACGCAATTA-CTCGAG-TAATTGCGTGTGCAGTGCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGCACTGCACACGCAATTACTCGAGTAATTGCGTGTGCAGTGCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGCACTGCACACGCAATTACTCGAGTAATTGCGTGTGCAGTGCAGT-3'

Other clones with same target sequence:

(none)