Construct: shRNA TRCN0000240026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001472641.1-307s21c1
DNA Barcode:
GTGGACATGTTAAGCGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm6158 (620499)
Gene Description:
predicted gene 6158
Transcript:
RefSeq XM_001472641.1 (NON-CURRENT)
Match location:
Position 307 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGACATGTTAAGCGAAATT
Hairpin Sequence:
5'-CCGG-GTGGACATGTTAAGCGAAATT-CTCGAG-AATTTCGCTTAACATGTCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGACATGTTAAGCGAAATTCTCGAGAATTTCGCTTAACATGTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGACATGTTAAGCGAAATTCTCGAGAATTTCGCTTAACATGTCCAC-3'

Other clones with same target sequence:

(none)