Construct: shRNA TRCN0000240037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_926073.2-1866s21c1
DNA Barcode:
AGTATACGTATTGGTTCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM49L2 (653111)
Gene Description:
tripartite motif containing 49-like 2
Transcript:
RefSeq XM_926073.2 (NON-CURRENT)
Match location:
Position 1866 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTATACGTATTGGTTCTTTA
Hairpin Sequence:
5'-CCGG-AGTATACGTATTGGTTCTTTA-CTCGAG-TAAAGAACCAATACGTATACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTATACGTATTGGTTCTTTACTCGAGTAAAGAACCAATACGTATACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTATACGTATTGGTTCTTTACTCGAGTAAAGAACCAATACGTATACT-3'

Other clones with same target sequence:

(none)