Construct: shRNA TRCN0000240086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001005103.2-352s21c1
DNA Barcode:
CTCCTTGGAGTCTTCTCATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC677582 (677582)
Gene Description:
similar to Dingo protein isoform 2
Transcript:
RefSeq XM_001005103.2 (NON-CURRENT)
Match location:
Position 352 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTTGGAGTCTTCTCATTC
Hairpin Sequence:
5'-CCGG-CTCCTTGGAGTCTTCTCATTC-CTCGAG-GAATGAGAAGACTCCAAGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTTGGAGTCTTCTCATTCCTCGAGGAATGAGAAGACTCCAAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTTGGAGTCTTCTCATTCCTCGAGGAATGAGAAGACTCCAAGGAG-3'

Other clones with same target sequence:

(none)