Construct: shRNA TRCN0000240123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_941485.2-1649s21c1
DNA Barcode:
GGATAGCAAGTCACTACTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC652147 (652147)
Gene Description:
u5 small nuclear ribonucleoprotein 200 kDa helicase-like
Transcript:
RefSeq XM_941485.2 (NON-CURRENT)
Match location:
Position 1649 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATAGCAAGTCACTACTATA
Hairpin Sequence:
5'-CCGG-GGATAGCAAGTCACTACTATA-CTCGAG-TATAGTAGTGACTTGCTATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATAGCAAGTCACTACTATACTCGAGTATAGTAGTGACTTGCTATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATAGCAAGTCACTACTATACTCGAGTATAGTAGTGACTTGCTATCC-3'

Other clones with same target sequence:

(none)